Our growing list of validated assays for routine screening includes:

Physicochemical properties • Kinetic solubility • Thermodynamic solubility • Lipophilicity    • Log D    • CHI •  Chemical stability Metabolic stability • Liver microsomes • P450 isoforms • UGT isoforms • Liver and intestinal S9 • Cryopreserved hepatocytes • Plasma stability Detailed metabolic studies • Metabolic identification • Reaction phenotyping • P450 spectral binding • Microsome kinetics

P450 inhibition • P450 isoform inhibition: fluorescence    or mass spectrometry based • Time dependant inhibition Distribution • Plasma protein binding • Blood partitioning • High-throughput permeability • PAMPA • Caco-2 permeation studies • P-gp ATPase assay Toxicity • Cytotoxicity using HepG2 cells • hERG inhibition Bioanalytical support • Method development • Dose formulation integrity testing • Quantification of compound in plasma    samples derived from PK or efficacy studies • Pharmacokinetic parameter calculation • Bioanalysis of tissue samples

The majority of these assays run in 96-well format using robotic handling with compound measurement by LC-MS/MS. All routine assays are performed accoring to validated, SOP controlled methods.

Specific protocols and additional assay formats can be adopted and validated to suit your project's needs.